Composite

Part:BBa_K5048072:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


T7 promoter-Neae-HSP60-FLAG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 92
    Illegal XhoI site found at 3872
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 2447
    Illegal AgeI site found at 2602
    Illegal AgeI site found at 3080
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1344


Design Notes

We constructed this part by restriction endonuclease cleavage and T4 ligation. During the process of design, we had to consider the enzyme and the cleavage site we needed to use.


Source

See our parts: BBa_I719005, BBa_K5048069


References